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  • arrchi
    Member
    • Mar 2011
    • 46

    cuffcompare <outprefix>.stats question

    Hi there,

    The values in the outprefix.stats of my cuffcompare are very low. Here is an example:

    #cuffcompare -V -r hg19.ensembl-for-tophat.gtf transcripts.gtf
    #

    #= Summary for dataset: transcripts.gtf :
    # Query mRNAs : 65495 in 64983 loci (5211 multi-exon transcripts)
    # (421 multi-transcript loci, ~1.0 transcripts per locus)
    # Reference mRNAs : 99330 in 43503 loci (82822 multi-exon)
    # Corresponding super-loci: 5232
    #--------------------| Sn | Sp | fSn | fSp
    Base level: 4.6 17.3 - -
    Exon level: 0.9 4.3 1.4 6.5
    Intron level: 2.5 80.2 2.5 81.7
    Intron chain level: 0.1 1.4 0.2 2.6
    Transcript level: 0.0 0.0 0.0 0.0
    Locus level: 0.2 0.1 0.2 0.2
    Missed exons: 333400/353286 ( 94.4%)
    Wrong exons: 56158/73680 ( 76.2%)
    Missed introns: 262639/272474 ( 96.4%)
    Wrong introns: 790/8364 ( 9.4%)
    Missed loci: 36101/43503 ( 83.0%)
    Wrong loci: 54293/64983 ( 83.5%)

    Total union super-loci across all input datasets: 59557
    Have anyone seen such low values? Does 94.4% means my data could not map to 94.4% of exons on my reference genome? What does "Wrong exons" mean?

    Thanks for any input!
    -A

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