Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • arrchi
    Member
    • Mar 2011
    • 46

    #1

    cuffcompare <outprefix>.stats question

    Hi there,

    The values in the outprefix.stats of my cuffcompare are very low. Here is an example:

    #cuffcompare -V -r hg19.ensembl-for-tophat.gtf transcripts.gtf
    #

    #= Summary for dataset: transcripts.gtf :
    # Query mRNAs : 65495 in 64983 loci (5211 multi-exon transcripts)
    # (421 multi-transcript loci, ~1.0 transcripts per locus)
    # Reference mRNAs : 99330 in 43503 loci (82822 multi-exon)
    # Corresponding super-loci: 5232
    #--------------------| Sn | Sp | fSn | fSp
    Base level: 4.6 17.3 - -
    Exon level: 0.9 4.3 1.4 6.5
    Intron level: 2.5 80.2 2.5 81.7
    Intron chain level: 0.1 1.4 0.2 2.6
    Transcript level: 0.0 0.0 0.0 0.0
    Locus level: 0.2 0.1 0.2 0.2
    Missed exons: 333400/353286 ( 94.4%)
    Wrong exons: 56158/73680 ( 76.2%)
    Missed introns: 262639/272474 ( 96.4%)
    Wrong introns: 790/8364 ( 9.4%)
    Missed loci: 36101/43503 ( 83.0%)
    Wrong loci: 54293/64983 ( 83.5%)

    Total union super-loci across all input datasets: 59557
    Have anyone seen such low values? Does 94.4% means my data could not map to 94.4% of exons on my reference genome? What does "Wrong exons" mean?

    Thanks for any input!
    -A

Latest Articles

Collapse

  • kinpict.com
    Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by kinpict.com
    The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
    Today, 06:37 PM
  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2





    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
    10-30-2026, 06:46 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 11-12-2026, 07:59 AM
0 responses
27 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-05-2026, 09:25 AM
0 responses
35 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-03-2026, 11:54 AM
2 responses
56 views
0 reactions
Last Post kinpict.com  
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
175 views
0 reactions
Last Post SEQadmin2  
Working...