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It seems like Artemis (release 12.0) translates DNA right ro protein, skipping mRNA stage, so that what seems 2nd reading frame of one chain is actually 2nd frame of complementary one. What is it: i'm wrong, or is this a bug, or is this a feature (and what is its function, if so)?
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Annotations created in Artemis represent the coding sequences of genes. So when the gene is annotated on the forward strand this means that the coding sequence is on the forward strand and that transcription of the gene occurs by reading the gene’s template sequence. So Artemis correctly shows the translation for the coding sequences.
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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