I just got back my Hi-Seq data which use CASAVA 1.8 to process the data and the quality scores are now in Sanger format Does anyone know a script which can transfer sanger fastQ into Illumina fastQ? thanks
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Since it took Illumina years to come to their senses about this and produce FASTQ in the accepted standard format can I ask why you want to go back the other way?Originally posted by sunsnow86 View Post
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our whole downstream analysis pipeline based on the Illumina score system, so it will be easier to convert it to that format.Comment
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You can use several existing tools to do the conversion to/from Illumina FASTQ and Sanger FASTQ, including EMBOSS seqret, Biopython, BioPerl, BioJava, BioRuby etc.Originally posted by sunsnow86 View Post
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