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  • Ami
    Junior Member
    • Aug 2010
    • 6

    #1

    Help with finding complementary sequences in ngs data

    Hello everybody,

    I am analyzing ngs data for small RNAs (18-30nt). I need to find out sequences which show complementarity for first 5-20 nt (from their 5' or 3' ends). I understand there are a lot of such sequences in my data set, after aligning them on to genome with Bowtie. I see many sequences that arise from plus strand, show overlap for 5-20 nt with the sequences arising from minus strand (complimentary). Please suggest me if there is any tool available to fish out this data .

    Thanks,

    Amit
  • StephaniePi83
    Member
    • Sep 2011
    • 52

    #2
    Hi Ami,
    did you find a tool able to do this ? I have the same thing to do !
    Thanks.

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