I have a small query regarding the whole genome re-sequencing of a human genome using SOLiD system from ABI. We have very little amount of DNA and we are able to only make 2 mate pair libraries form it(25 microgram each). Our main interest is to detect genetic variations. We are planning to run 4 slides from one library,pulling it form 8 ePCRs(we will club 2 ePCR products to make one slide) so that we utilize maximum number of beads. We are expecting 8x-10x coverage after running both the libraries. Now I would like to know, is is sufficient coverage to determine true variants and other variation like indel? If it is sufficient coverage what is suggested threshold to call SNPs?
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM