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what is the difference between exome and transcriptome ? how does their assembly differ ?
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is it transcriptome is always time specific whereas exome is whole rna count irrespective of the time? -
The transcriptome consists of all that is transcribed, ie all RNA species including mRNA, miRNA, tRNA, lncRNA, etc. It is usually studied through samples of RNA. You can get the basic idea by reading this: http://en.wikipedia.org/wiki/Transcriptome
The exome refers to the protein coding portion of the genome. It is usually studied through samples of DNA. I'd recommend reading this article for an example of exome sequencing and its applications: http://www.ncbi.nlm.nih.gov/pmc/arti...1/?tool=pubmedComment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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