I tried to use mpileup in samtools to call SNP and indel from 200 individuals of whole genome sequencing. However, it is a time consuming job, in about 10 days, only the variants in three chromosomes have been outputted in the bcf file. So, if the mpileup can make variants call separately in chromosomes, I can run multiple chromosomes at the same time on my computer. But the parameter -l need the user to provide candidate variants position.Does anyone have some experence in this situation?
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
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