Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Coverage statistics on single exons

    Hi,

    I'm engaged in an exome sequencing project.
    I have done my NGS exome sequencing using illumina platform with an average 50X coverage and the Agilent SureSelect capture kit.
    I've aligned all the reads and generated the corresponding bam file. Now I'm doing same data analysis but visually inspecting the aligned reads, I've noted that some exons (from RefSeq) seem to have no coverage at all.
    So I'm interested in a report on exact coverage for the various exons. In particular is there a way to know if there are exons with no coverage, how many of them and eventually which ones?

    Thanks,
    Edoardo

Latest Articles

Collapse

  • seqadmin
    Best Practices for Single-Cell Sequencing Analysis
    by seqadmin



    While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
    06-06-2024, 07:15 AM
  • seqadmin
    Latest Developments in Precision Medicine
    by seqadmin



    Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

    Somatic Genomics
    “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
    05-24-2024, 01:16 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 06:58 AM
0 responses
13 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-06-2024, 08:18 AM
0 responses
20 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-06-2024, 08:04 AM
0 responses
18 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-03-2024, 06:55 AM
0 responses
13 views
0 likes
Last Post seqadmin  
Working...
X