Dear colleagues, I have implemented two software packages. HighSSR framework can significantly simplify development of the microsatellites (SSR) markers from the next generation sequencing reads (especially from Roche 454 GS-FLX raw reads because they are longer). The other package is for Illumina RNA-Seq transcriptome analysis in case/control studies. These are freely available from here http://maximusinuse.nmsu.edu. Please let me know your opinion about these developments, we will submit corresponding method papers for review shortly.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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