Tweet
How to make mates file from the velvet contig output for bambus. The IID of velvet contig file(convert by amos package) is always the numbers, how can we tell the paired-end sequence from this file?
Thanks!
Thanks!
-
-
Hi,Originally posted by elisadouzi View Post
I am also trying to do the same. Have you already generated the bambus2 required input files(.contig and .mate)? I have .contig file from the minimus2 run, But I am not sure how to get .mate file. Can I generate it from the Illumina paired-end reads information? the reads I assembled??
Best regards,
RahulRahul Sharma,
Ph.D
Frankfurt am Main, Germany -
Same here!
Bambus2 within AMOS did not work for me either. Is it possible to open an a thread in this forum?Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment