Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • zlix425
    Junior Member
    • Jul 2011
    • 1

    #1

    Problems with understanding Quake's outputs

    Hi, I am new to this area and I am currently working on Illumina GA data analysis. I have this output from the software Quake:

    ...3598636 sequences processed, 359863600 bp scanned
    WARNING: Input had 352880 non-DNA (ACGT) characters whose kmers were not counted
    11120988 total distinct mers
    11120988 mers occur at least 0 times
    initial value 63013.294557
    iter 10 value 57511.692087
    iter 20 value 56339.034500
    iter 30 value 56016.040353
    iter 40 value 56012.926228
    iter 50 value 56011.300715
    final value 56010.011528
    converged
    value: 56010.01
    $zp.copy
    [1] 3.078884

    $p.e
    [1] 0.715543

    $shape.e
    [1] 0.1971554

    $scale.e
    [1] 1.328558

    $u.v
    [1] 57.375

    $var.v
    [1] 880.9596

    I am not sure if the "warning" produced above affects the results? Also, I am not quite sure what are the meanings of "iter" values above as I could not find the meaning of these terms from the literature.

    Thanks for anyone that could help.
  • ECO
    --Site Admin--
    • Oct 2007
    • 1360

    #2
    Don't crosspost. Your other post was deleted. One post, one location.

    Comment

    Latest Articles

    Collapse

    • kinpict.com
      Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by kinpict.com
      The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
      Today, 06:37 PM
    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2





      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
      10-30-2026, 06:46 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 11-12-2026, 07:59 AM
    0 responses
    27 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 11-05-2026, 09:25 AM
    0 responses
    35 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 11-03-2026, 11:54 AM
    2 responses
    56 views
    0 reactions
    Last Post kinpict.com  
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    175 views
    0 reactions
    Last Post SEQadmin2  
    Working...