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  • wanguan2000
    Member
    • Nov 2010
    • 24

    GATK IndelGenotyperV2 ERROR

    when I followed the pipeline:


    java -Xmx4g -jar GenomeAnalysisTK.jar \
    -l INFO \
    -nt 8 \
    -R ./hg19fasta/ucsc.hg19.fasta \
    -B:dbsnp,vcf /share/user_data/wfz1/database/hg19database/dbsnp_132.hg19.vcf \
    -I ./hg19fasta/pair-end32.sorted.bam \
    -T CountCovariates \
    -cov ReadGroupCovariate \
    -cov QualityScoreCovariate \
    -cov CycleCovariate \
    -cov DinucCovariate \
    -recalFile ./hg19fasta/32.csv \
    --default_read_group HWI-ST499:5:5:8126:176904#CTTGTA \
    --default_platform illumina

    .......
    in this step:

    java -Xmx4g -jar GenomeAnalysisTK.jar \
    -T IndelGenotyperV2 \
    -l INFO \
    -R ./hg19fasta/ucsc.hg19.fasta \
    -I ./hg19fasta/cleaned31.bam \
    -bed my.brief.output.bed \
    -o my.detailed.output.vcf

    I got this ERROR:
    Badly formed genome loc: Parameters to GenomeLocParser are incorrect:The stop position 1 is less than start 150
    what is problem? I'm just following the pipeline.
    ps:my bam data is : pair-end31.sorted.bam
    Last edited by wanguan2000; 07-24-2011, 06:39 PM.
  • wanguan2000
    Member
    • Nov 2010
    • 24

    #2
    java -Xmx4g -jar /share/user_data/wfz1/wangguan/GATK/GenomeAnalysisTK-1.1-3-g1f8fc4a/GenomeAnalysisTK.jar \
    -T SomaticIndelDetector \
    -l INFO \
    -R ./hg19fasta/ucsc.hg19.fasta \
    -I ./hg19fasta/cleaned31.bam \
    -bed ./hg19fasta/my.brief.output31.bed\
    -verbose ./hg19fasta/my.detailed.output31.txt \
    -o ./hg19fasta/my.output31.vcf

    GenomeAnalysisTK-1.1-3-g1f8fc4a don't contain the IndelGenotyperV2, It has the SomaticIndelDetector.
    in my case, the pair-end data was merger in one pair-end31.sam. The SomaticIndelDetector was need -I:normal data1.bam -I:tumor data2.bam.
    what can I do?

    Comment

    • RDW
      Member
      • Oct 2008
      • 63

      #3
      'Paired sample mode' in the Somatic Indel Detector docs refers to tumour/normal pairs. If you don't have these, you can follow the instructions for 'Single Sample Mode' (the single input bam file can contain paired end data). However, in this case you'd be much better off following the current pipeline:



      This uses the Unified Genotyper to call both indels and SNPs. For help with this, see:



      The old v1 pipeline you were using is not designed to work with recent versions of the GATK.

      Comment

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