some silly questions:
1. -F FLOAT minimum fraction of gapped reads for candidates [0.002]
what's the meaning of gapped reads? does that mean the reads are juntion reads?
for example:B810KBABXX:8:27:6656:80899#ACAGTGAT 99 chr1 509019 255 76M2010N24M = 511155 0 TCTCCTCCAAGACAACCCGCTGTCCGTCCAGACTCGATATAGGGGCTCCGAGGTCCAGGGGCTCCTTCTCTCCACTCAGCCTCACCAGCAGGCCACTGAG FFF+FGGGGGHHFHHHHHHHHFCHHHFH?HCGFDGEEEFEHDHHBFEFHB9AD<DFDEEBE:EEEEHEEECEEB@DGEEFH?HFFHBBHFEHCF9:/:76 NM:i:0 XS:A:- NH:i:1
2.are there any parameters to filter or control the mismatch near to or just in the end of a read?
3.what's the meaning of 'cap mapping quality'?
1. -F FLOAT minimum fraction of gapped reads for candidates [0.002]
what's the meaning of gapped reads? does that mean the reads are juntion reads?
for example:B810KBABXX:8:27:6656:80899#ACAGTGAT 99 chr1 509019 255 76M2010N24M = 511155 0 TCTCCTCCAAGACAACCCGCTGTCCGTCCAGACTCGATATAGGGGCTCCGAGGTCCAGGGGCTCCTTCTCTCCACTCAGCCTCACCAGCAGGCCACTGAG FFF+FGGGGGHHFHHHHHHHHFCHHHFH?HCGFDGEEEFEHDHHBFEFHB9AD<DFDEEBE:EEEEHEEECEEB@DGEEFH?HFFHBBHFEHCF9:/:76 NM:i:0 XS:A:- NH:i:1
2.are there any parameters to filter or control the mismatch near to or just in the end of a read?
3.what's the meaning of 'cap mapping quality'?