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Does gene fusion happen at genomic level or transcript level ? what is the difference between fusion genes and chimeric transcripts, are they the same?
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In answer to your first question, both. Most fusions of biological interest appear to be driven by genomic rearrangements. However, there are definitely examples in which mutations in splicing or polyadenylation signals lead to the formation of fusion transcripts from unrearranged (but adjacent) genes. -
We have recently sequenced a bacterial transcriptome with 316 chip from IonTorrent (1.5 million sequences). After filtering low quality data and trimming adapters we noticed that only 51.33% sequences were mapped on reference genome. Looking for the unmapped sequences we can see that most of them are chimeric transcripts, so impossible mapping for them an also causing bias on results. Also many of the unmapped are sequences lacking homology in 20% of the starting sequence.
I would like to know your opinion about it.
Should I have to move to 454 or Illumina? Our Sequencing Department have no idea of why we have so many chimeras.
Thank you, BernardoComment
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I would recommend illumina as it has more advanced and standardized protocol with high accuracy. Or u can refer
"Direct Comparisons of Illumina vs. Roche 454 Sequencing Technologies on the Same Microbial Community DNA Sample" at
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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