Dear all,
As per the title, I have 48 small RNA samples that I've run as part of a barcoded experiment. I've downloaded the primary data which includes the cfasta and qual files for analysis.
My interest is miRNAs and I understand this area is still very new and ever changing.
Just wondering whether anyone has any recommendations for analysis software. I've run the samples through CLC Bio but having got raw miRNA counts, I am unsure how to procede with normalising these values (my various libraries have very different total counts in some cases as they come from different tissue pathologies).
Moreover, CLC doesn't give me the aligned reads in a format I can easily manage downstream.
I'm therefore looking for a complete solution from csfasta to normalised vaules...
Any help would be much appreciated.
D
As per the title, I have 48 small RNA samples that I've run as part of a barcoded experiment. I've downloaded the primary data which includes the cfasta and qual files for analysis.
My interest is miRNAs and I understand this area is still very new and ever changing.
Just wondering whether anyone has any recommendations for analysis software. I've run the samples through CLC Bio but having got raw miRNA counts, I am unsure how to procede with normalising these values (my various libraries have very different total counts in some cases as they come from different tissue pathologies).
Moreover, CLC doesn't give me the aligned reads in a format I can easily manage downstream.
I'm therefore looking for a complete solution from csfasta to normalised vaules...
Any help would be much appreciated.
D
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