Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • honey
    Senior Member
    • Feb 2010
    • 151

    #1

    some odd out put in combined GTF RNA-seq Cufflink

    I am using the most recent version of Cufflink and has used the previous version of same tool for RNA-seq analysis. After updating new version I ran few RNA-seq analysis and have noted one strange point in combined GTF files out put;
    In column old cufflink Ids there are few entries which are repeated for Ensembl transcript ids meaning same Ensembl transcript id is put in two columns one under olds cufflink id and second under Ensembl transcript id. There are lot of such entries. I was wondering if someone else have experienced the same pattern or I messed up something? ( I think I am not)
    Old cufflink ids used to be unique per row, then in this scenario how should be interpret? I checked these ENST repeated entries back into Ensembl they are not unidentified and are well defined?

    Thanks for your input

Latest Articles

Collapse

  • SEQadmin2
    Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
    by SEQadmin2



    CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

    Despite this, “CRISPR helped turn genome editing from a specialized technique into
    ...
    07-31-2026, 11:01 AM
  • SEQadmin2
    Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
    by SEQadmin2


    Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

    The systematic characterization of the human proteome has
    ...
    07-20-2026, 11:48 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Yesterday, 12:22 PM
0 responses
12 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 08-11-2026, 10:35 AM
0 responses
13 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 08-06-2026, 07:41 AM
0 responses
31 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 08-03-2026, 10:13 AM
0 responses
48 views
0 reactions
Last Post SEQadmin2  
Working...