Hi all,
Does anybody know whether it will be kosher to use the default settings of fastq-dump (Q=33) for Illumina datasets ? I am not sure whether the original Illumina reads are generated using the Sanger offset or not. Would it even make a difference to use the default Q setting for converting Illumina sra data into fastq format?
Thank you!
Duygu
Does anybody know whether it will be kosher to use the default settings of fastq-dump (Q=33) for Illumina datasets ? I am not sure whether the original Illumina reads are generated using the Sanger offset or not. Would it even make a difference to use the default Q setting for converting Illumina sra data into fastq format?
Thank you!
Duygu
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