Tweet
anyone knows about how to do that?
Thanks!
Thanks!
-
-
No need to do so. The libraries are already extracted by the machine, at least on the SOLiD 4 and SOLiD 3. -
really?I just got one csfasta and qual file including 8 libraries from the core facility. If so, I will ask them tomorrow.Comment
-
Yes, ask them. Let us know what they say. Since the SOLiD has separate runs for the barcode, the F3 and the F5/R3 then these should separate into three different files and into different samples. We get (and give to our customers) files that are named like:
run00165_Stemredo_BC_000738_StemRedo_05.csfasta
run00165_Stemredo_F5-BC_000738_StemRedo_05.csfasta
run00165_Stemredo_F3_000738_StemRedo_05.csfasta
run00165_Stemredo_BC_000739_StemRedo_06.csfasta
run00165_Stemredo_F5-BC_000739_StemRedo_06.csfasta
run00165_Stemredo_F3_000739_StemRedo_06.csfasta
Plus associated qual and stats files; e.g.,
run00165_Stemredo_BC_QV_000738_StemRedo_05.qual
run00165_Stemredo_BC.stats
Note that there are three .csfasta files per sample.
It is possible to merge all data from the samples into one file. But, at the risk of critiquing another core facility, why do so?Comment
-
I'm having the same problem. I have four barcoded samples, but only got one csfasta and qual files. Did the ppl got the solution for the problem from ABI?
Thx!Comment
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Comment