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  • lei
    Member
    • Mar 2010
    • 14

    #1

    how to trim solid reads length?

    are there any tools available to trim solid reads length? for example, I want to extract first 30bp.
    Thanks!
  • westerman
    Rick Westerman
    • Jun 2008
    • 1104

    #2
    No tools that I know of. Both Emboss and Galaxy do not seem to have a way (although there are so many programs in both that it is hard to tell.) Trimming is trivial to do in perl or another language. If you are a "layer 3" bioinformatics person (see http://www.homolog.us/blogs/?p=85) then it will take less time to write the program than to respond back to this message. :-)

    Comment

    • lei
      Member
      • Mar 2010
      • 14

      #3
      OK, I wrote one, but not so fast, that's why I ask here to check whether there is some tool to do it already.

      Comment

      • gringer
        David Eccles (gringer)
        • May 2011
        • 845

        #4
        I've managed to do it by double-encoding, passing through the fastx-toolkit, then reversing the double-encoding.

        Comment

        • carmeyeii
          Senior Member
          • Mar 2011
          • 137

          #5
          Are there any new ways to do this?

          Comment

          • gsgs
            Senior Member
            • Oct 2009
            • 139

            #6
            I don't understand. "trim" = cut ? zurechtstutzen
            Nor do I know/understand Emboss,Galaxy,layer 3,
            double-encoding,fastx.
            I just feel, there must be something wrong with bioinformatics,
            if cutting the length of a sequence gets a thread here.

            Comment

            • gringer
              David Eccles (gringer)
              • May 2011
              • 845

              #7
              Originally posted by gsgs View Post
              I just feel, there must be something wrong with bioinformatics,
              if cutting the length of a sequence gets a thread here.
              Let's reiterate what westerman said:

              Originally posted by westerman
              Trimming is trivial to do in perl or another language.... it will take less time to write the program than to respond back to this message.
              Or, for that matter, reading through all the posts in this thread, then asking if anyone has thought up their new favourite way to trim reads to a particular length.

              Comment

              • carmeyeii
                Senior Member
                • Mar 2011
                • 137

                #8
                Lovely

                Cheers.

                Comment

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