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I am dealing a set of RNA-seq data generated by Illumina, aligned and assembled by tophat-cufflinks pipeline. I wonder why so many of my non-code RNA expression values are zero, but not all of them?
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I"m guessing this is, for you, an early look at rnaseq results. Some genes are expressed, some aren't; this includes non-coding RNA. You'll see this as aligned reads with a gene (mostly the exons) and some genes have no alignments (meaning , likely, that they are not expressed or got filtered out during wet-lab processing). Some protocols only pick up polya mrna. If you can fully explain why some are lighting up and others aren't; then you got a paper.
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Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.
Nobel Prize for MicroRNA Discovery
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Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...09-23-2024, 06:35 AM
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