I am dealing a set of RNA-seq data generated by Illumina, aligned and assembled by tophat-cufflinks pipeline. I wonder why so many of my non-code RNA expression values are zero, but not all of them?
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I"m guessing this is, for you, an early look at rnaseq results. Some genes are expressed, some aren't; this includes non-coding RNA. You'll see this as aligned reads with a gene (mostly the exons) and some genes have no alignments (meaning , likely, that they are not expressed or got filtered out during wet-lab processing). Some protocols only pick up polya mrna. If you can fully explain why some are lighting up and others aren't; then you got a paper.
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The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.Today, 06:37 PM -
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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