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  • fabrice
    Member
    • Oct 2009
    • 86

    #1

    From sam file, how to the mate aligned or not?

    For paired-end data, which flag to indicate the mate aligned or not?

    Thanks.
  • swbarnes2
    Senior Member
    • May 2008
    • 910

    #2
    If the 8 flag is set, the mate didn't map. It's right there in the .sam format description.

    Comment

    • fabrice
      Member
      • Oct 2009
      • 86

      #3
      PNEXT: Position of the NEXT fragment in the template. Set as 0 when the information is
      unavailable. This eld equals POS of the next fragment. If PNEXT is 0, no assumptions can be
      made on RNEXT and bit 0x20.

      If the 8 flag is set, the mate map?

      Originally posted by swbarnes2 View Post
      If the 8 flag is set, the mate didn't map. It's right there in the .sam format description.

      Comment

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