I tried to run TopHat Fusion on canine mRNA-seq data (Illumina paired-end), but received the error below.
Perhaps it is an inappropriate use of the software (giving it RNA data instead of DNA data)?
Any other ideas? Anyone else gotten this error or tried using Tophat Fusion in this way?
[Wed Aug 10 04:40:50 2011] Joining segment hits
Traceback (most recent call last):
File "./tophatfusion-0.1.0-bin/tophat-fusion", line 2610, in ?
sys.exit(main())
File "./tophatfusion-0.1.0-bin/tophat-fusion", line 2566, in main
user_supplied_deletions)
File "./tophatfusion-0.1.0-bin/tophat-fusion", line 2255, in spliced_alignment
right_reads_map = maps[right_reads].unspliced_bwt
AttributeError: 'list' object has no attribute 'unspliced_bwt'
Perhaps it is an inappropriate use of the software (giving it RNA data instead of DNA data)?
Any other ideas? Anyone else gotten this error or tried using Tophat Fusion in this way?
[Wed Aug 10 04:40:50 2011] Joining segment hits
Traceback (most recent call last):
File "./tophatfusion-0.1.0-bin/tophat-fusion", line 2610, in ?
sys.exit(main())
File "./tophatfusion-0.1.0-bin/tophat-fusion", line 2566, in main
user_supplied_deletions)
File "./tophatfusion-0.1.0-bin/tophat-fusion", line 2255, in spliced_alignment
right_reads_map = maps[right_reads].unspliced_bwt
AttributeError: 'list' object has no attribute 'unspliced_bwt'
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