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  • Palgrave
    Member
    • Aug 2011
    • 73

    #1

    filtering small RNA TruSeq reads

    I just got smallRNA-seq reads prepared by the TruSeq small RNA protocol from Illumina with index 1-7 and I am wondering what filtration is necessary?

    I have started by removing the revers complement of the following PCR primer:

    RNA PCR Primer, Index 1 (RPI1)
    CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA


    Do I need to remove anything in addition, like any of these:

    RNA 5’ Adapter (RA5), part # 15013205
    5’ GUUCAGAGUUCUACAGUCCGACGAUC

    RNA 3’ Adapter (RA3), part # 15013207
    5’ TGGAATTCTCGGGTGCCAAGG

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