I have got the raw counts for each exon using HTSeq-count. I want to use DESeq to normalise the counts. How can I output the Normalised count data from DESeq?
Unconfigured Ad
Collapse
X
-
cds <- newCountDataSet( countsTable, conds )
cds <- estimateSizeFactors( cds )
normalizedCounts <- t( t(counts(cds)) / sizeFactors(cds) )
look at the DESeq vignette for more infos : http://bioconductor.org/packages/2.8.../doc/DESeq.pdfLast edited by NicoBxl; 08-16-2011, 11:04 PM.
Latest Articles
Collapse
-
by SEQadmin2
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...-
Channel: Articles
-
ad_right_rmr
Collapse
News
Collapse
| Topics | Statistics | Last Post | ||
|---|---|---|---|---|
|
Started by SEQadmin2, Today, 11:14 AM
|
0 responses
8 views
0 reactions
|
Last Post
by SEQadmin2
Today, 11:14 AM
|
||
|
Started by SEQadmin2, 09-29-2026, 09:51 AM
|
0 responses
44 views
0 reactions
|
Last Post
by SEQadmin2
09-29-2026, 09:51 AM
|
||
|
Started by SEQadmin2, 09-25-2026, 09:06 AM
|
0 responses
53 views
0 reactions
|
Last Post
by SEQadmin2
09-25-2026, 09:06 AM
|
||
|
Started by SEQadmin2, 09-23-2026, 11:05 AM
|
0 responses
44 views
0 reactions
|
Last Post
by SEQadmin2
09-23-2026, 11:05 AM
|
Comment