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  • feng
    Member
    • Oct 2010
    • 50

    #1

    bacterial DNA databases

    Hi everyone, Do you know where I can find best bacterial DNA sequences?

    NCBI or MGDB,

    and How to get all these reads?

    Many thanks, Feng
  • westerman
    Rick Westerman
    • Jun 2008
    • 1104

    #2
    For NCBI go to the genome section. From there it should be obvious how to download the reads. Or you could read the help documents if you need more help.

    Download a genome data package including genome, transcript and protein sequence, annotation and a data report

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    • GenoMax
      Senior Member
      • Feb 2008
      • 7142

      #3
      Also check out CMR at JCVI: http://cmr.jcvi.org/tigr-scripts/CMR/CmrHomePage.cgi
      JGI Genome Projects: http://www.jgi.doe.gov/genome-projects/

      Comment

      • gsgs
        Senior Member
        • Oct 2009
        • 139

        #4
        I had downloaded the 85 bacterial files from genbank release 189
        that was 19GB decompressed

        created the fasta from it

        ~5 hours

        sort by species, create sub-databases, make consensus/index sequences,
        align, list sequances by changes only
        publish the compressed aligned species-subdatabases !

        18000 bacteria, 823012 entries, amino-acid sequences ~2GB
        I must have a thread at flutrackers


        most frequent:
        46323, Escherichia coli
        20346, Helicobacter pylori
        15134, Bacillus
        13565, Listeria monocytogenes
        13486, Vibrio
        13331, Pseudomonas
        13249, bacterium
        11046, Neisseria meningitidis
        11033, Mycobacterium
        09981, Streptomyces
        08615, Streptococcus pneumoniae
        07850, Bradyrhizobium
        07747, Salmonella entericasu
        07012, Staphylococcus aureus
        06620, Borrelia burgdorferi
        05808, Bacillus subtilis
        05547, Vibrio cholerae
        05139, Clostridium perfringens
        04547, Bacillus cereus
        04479, Pseudomonas aeruginosa
        04475, Streptococcus mutans
        04319, Burkholderia
        04308, Ralstonia solanacearum
        04065, Chlamydia trachomatis
        03992, Streptococcus mitis
        03873, Haemophilus influenzae
        03452, Legionella pneumophila
        03405, Klebsiella pneumoniae
        03335, Rhizobium
        03229, Streptococcus pyogenes
        03009, Bacillus thuringiensis
        02997, Campylobacter jejuni
        02969, Pseudomonas syringae
        02876, Arthrobacter
        Last edited by gsgs; 12-01-2012, 07:28 PM.

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