Hi,
I've got some sequencing data which following DE analysis with DESeq gives p-values for many genes of < 1x10-80. Is this typical? How does DESeq generate such small p-values where I don't think there's enough information to do so? Does this mean that using a p-value cut-off of 0.05 or 0.01 is too lenient? Or am I missing something.
The data is four replicates of one condition versus 6 replicates of another sequenced with direct RNA seq.
Any clarification much appreciated.
I've got some sequencing data which following DE analysis with DESeq gives p-values for many genes of < 1x10-80. Is this typical? How does DESeq generate such small p-values where I don't think there's enough information to do so? Does this mean that using a p-value cut-off of 0.05 or 0.01 is too lenient? Or am I missing something.
The data is four replicates of one condition versus 6 replicates of another sequenced with direct RNA seq.
Any clarification much appreciated.
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