Thanks vebaev,
Do you use Deseq for miRNA expression analysis?
One of my concern is the number of miRNA I have in my list is small, 300 ~ 800, depending on a category.
Can I assume that most of miRNA does not change expression levels as we do in mRNA expression analysis?
I think Deseq is good for gene expression analysis, ie. most of genes in some thousands of transcripts do not show expression changes. But I'm not sure miRNA expression analysis adopt this hypothesis.
-
microRNA normalization
Hi,
Does anyone know the reasonable normalization methods for microRNA data analysis?
I already trimmed adaptor, mapped these reads to the reference, and counted the number of reads that mapped on the known microRNA seq.
What I have is a list of known mature microRNAs' ID and sequence, and the number of reads mapped to this miRNAs.
I have 5 samples and want to compare differential expression among them.
How do you normalize the number of mapped reads in order to compare the results from different samples?
Any RPKM-like methods?
Thanks,
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Leave a comment: