Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Regarding fastq file format

    I downloaded a file from 1000 genomes.
    ftp://ftp.1000genomes.ebi.ac.uk/vol1....filt.fastq.gz

    The contents look like this:

    @SRR040813.1390 VAB_0100_20091102_2_SL_AWG_TG_NA19150_000pA_01003244543_12_21_53/1
    G22300322011100202023200032002..200.330022102.22002
    +
    !'1)#$)&5&'$''%''3&'&,24)&*&2,!!',4!11,4)&&8%!),#52
    @SRR040813.1391 VAB_0100_20091102_2_SL_AWG_TG_NA19150_000pA_01003244543_12_21_64/1
    G20221312101332122023110001200..200..10032100.20000
    +
    !#)(+)(0+%))3&*)&0&+1(&&&)'2:7!!66(!!845/)<8.!.&)5)


    As per my knowledge, the second line should be a sequence of A,T, G, C, N. Is this a corrupted file or is it a different way of representing the gene data in fastq file?

  • #2
    Looks like Colorspace:
    Generated by all sequencers from the solid and 5500ish sequencers sequencers from ABI. The numbers represent colors instead of actual bases while the dot represents positions where no actual color read could be obtained. See this paper for an overview of the technology: http://www.ncbi.nlm.nih.gov/pubmed/19997069

    Hope that helps

    Comment


    • #3
      Thanks a lot peter

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Understanding Genetic Influence on Infectious Disease
        by seqadmin




        During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.

        Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...
        09-09-2024, 10:59 AM
      • seqadmin
        Addressing Off-Target Effects in CRISPR Technologies
        by seqadmin






        The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...
        08-27-2024, 04:44 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Today, 06:25 AM
      0 responses
      13 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, Yesterday, 01:02 PM
      0 responses
      12 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 09-18-2024, 06:39 AM
      0 responses
      14 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 09-11-2024, 02:44 PM
      0 responses
      14 views
      0 likes
      Last Post seqadmin  
      Working...
      X