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  • Regarding fastq file format

    I downloaded a file from 1000 genomes.
    ftp://ftp.1000genomes.ebi.ac.uk/vol1....filt.fastq.gz

    The contents look like this:

    @SRR040813.1390 VAB_0100_20091102_2_SL_AWG_TG_NA19150_000pA_01003244543_12_21_53/1
    G22300322011100202023200032002..200.330022102.22002
    +
    !'1)#$)&5&'$''%''3&'&,24)&*&2,!!',4!11,4)&&8%!),#52
    @SRR040813.1391 VAB_0100_20091102_2_SL_AWG_TG_NA19150_000pA_01003244543_12_21_64/1
    G20221312101332122023110001200..200..10032100.20000
    +
    !#)(+)(0+%))3&*)&0&+1(&&&)'2:7!!66(!!845/)<8.!.&)5)


    As per my knowledge, the second line should be a sequence of A,T, G, C, N. Is this a corrupted file or is it a different way of representing the gene data in fastq file?

  • #2
    Looks like Colorspace:
    Generated by all sequencers from the solid and 5500ish sequencers sequencers from ABI. The numbers represent colors instead of actual bases while the dot represents positions where no actual color read could be obtained. See this paper for an overview of the technology: http://www.ncbi.nlm.nih.gov/pubmed/19997069

    Hope that helps

    Comment


    • #3
      Thanks a lot peter

      Comment

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