Errors encountered while running Metasim

[sandhya]

Hi,

I am using Metasim to simulate 454 reads. This is the 454 error model setting.

Preset Name: 454
Number Of Reads / Mate Pairs=5000
Error Model=454

454 Error Model Configuration=
Number Of Cycles: 133 (~339 Base Pairs)
Mate Pair Probability: 0.0
Mate Pair Read Length: 20
Remove Mate Pair Linker from Output: false
Lognormal Distribution Mean: 0.23
Lognormal Distribution Std. Deviation: 0.15
Proportionality Constant for Std. Deviation: 0.15
Scale Std. Deviation with Square Root of Mean: true
Generate Signal Trace: false

454 Error Model DNA Clone Parameters=
Distribution: Normal
Mean: 400.0
2nd parameter: 20.0

Combine All Files=false
Uniform Sequence Weights=false
Number Of Threads=1
Write FastA=true
Compress Output Files=true

The Taxon profile is

### MetaSim taxon profile
# 100 name "07-54825"
100 taxid 1

I run into this error (this is from the Simulator log file)

Thu Sep 01 15:16:50 CEST 2011

Simulating files:
[11.mprf]

Simulator Settings:
Preset Name: 454
Number Of Reads / Mate Pairs=5000
Error Model=454

454 Error Model Configuration=
Number Of Cycles: 133 (~339 Base Pairs)
Mate Pair Probability: 0.0
Mate Pair Read Length: 20
Remove Mate Pair Linker from Output: false
Lognormal Distribution Mean: 0.23
Lognormal Distribution Std. Deviation: 0.15
Proportionality Constant for Std. Deviation: 0.15
Scale Std. Deviation with Square Root of Mean: true
Generate Signal Trace: false

454 Error Model DNA Clone Parameters=
Distribution: Normal
Mean: 400.0
2nd parameter: 20.0

Combine All Files=false
Uniform Sequence Weights=false
Number Of Threads=1
Write FastA=true
Compress Output Files=true


+++ File: 11.mprf +++
java.lang.Exception: sequence file not found
!!! FAILED !!!

Please advise if I have missed anything in the settings or why this error possibly occurs.

Thank you.

[beacamara]

Hi sandhya!

Could you fix the error exception raise from MetaSim?
It's happening to me now, after using MetaSim almost a year.

Thank you so much

Bea