So, I'm working with a several bacterial genomes, and I'm learning bioinformatics as I work, so forgive me if this is a dumb question. My genomes have a lot of repeat sequences (for a prokaryotic genome) that are really the last elements to place into the genome, however I want to make sure that I assemble everything correctly. Thus, my question is how do you make sure that repeat sequences are placed correctly in the genome. Is the best way to PCR out from the repeat sequences and see where the different sequences belong, or are there other easier and quicker ways to accomplish this goal. Basically, what is the fastest and most accurate way to determine the location of the repeat sequences. Sorry again if this is an dumb question, but I don't know that much about genome sequencing and bioinformatics and I'm having to learn a lot from everyone here. Thanks
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Last edited by clostridium40; 09-02-2011, 01:25 PM.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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