So, I'm working with a several bacterial genomes, and I'm learning bioinformatics as I work, so forgive me if this is a dumb question. My genomes have a lot of repeat sequences (for a prokaryotic genome) that are really the last elements to place into the genome, however I want to make sure that I assemble everything correctly. Thus, my question is how do you make sure that repeat sequences are placed correctly in the genome. Is the best way to PCR out from the repeat sequences and see where the different sequences belong, or are there other easier and quicker ways to accomplish this goal. Basically, what is the fastest and most accurate way to determine the location of the repeat sequences. Sorry again if this is an dumb question, but I don't know that much about genome sequencing and bioinformatics and I'm having to learn a lot from everyone here. Thanks
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Last edited by clostridium40; 09-02-2011, 01:25 PM.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM