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i am running cufflinks as:
cufflinks -p 2 -o cuff_1.out -G ~/harsh_ngs/human_ref/human_protein_coding.gtf -g ~/harsh_ngs/human_ref/human_protein_coding.gtf -b ~/harsh_ngs/human_ref/BowtieIndex/genome.fa -u --library-type fr-unstranded -F 0.25 -j 0.30 ../accepted_hits.bam
i have edited the ensembl gtf file to make it compatible with cufflinks, my gtf file looks something like as mentioned in attachment.
while running above cufflinks command i am getting error as
Loading reference annotation.
Segmentation fault
is there a error in gtf file or in my command?
cufflinks -p 2 -o cuff_1.out -G ~/harsh_ngs/human_ref/human_protein_coding.gtf -g ~/harsh_ngs/human_ref/human_protein_coding.gtf -b ~/harsh_ngs/human_ref/BowtieIndex/genome.fa -u --library-type fr-unstranded -F 0.25 -j 0.30 ../accepted_hits.bam
i have edited the ensembl gtf file to make it compatible with cufflinks, my gtf file looks something like as mentioned in attachment.
while running above cufflinks command i am getting error as
Loading reference annotation.
Segmentation fault
is there a error in gtf file or in my command?
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