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  • phatjoe
    Member
    • Aug 2011
    • 13

    #1

    BFAST indexing

    Hi,
    I've recently been introduced to BFAST. So please bear with me.

    I am trying on reads from Illumina (36bp) but how is the -m and -w option determined?

    With the reads of <40bp, my reads should best have the key size of 18. I see variations of -m options with 111111111111111111,11111111111111111101 ,1111101110111010100101011011111 and etc. Can someone please explain to me the difference of it?

    Thanks in advance!
  • nilshomer
    Nils Homer
    • Nov 2008
    • 1283

    #2
    The appendix states that you should make ten indexes, so you need to have ten "bfast index" commands, one for each mask.

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