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  • Reading run reports and other documents

    Hi all,

    I've written a bunch of little reports relating to next-gen sequencing. Many of them used to be hosted as Genographia but I've moved them to my personal wiki here:



    I've also included a short writeup on Genome Analyzer run reports and how to read them: http://sgenomics.org/mediawiki/index..._runreport.pdf which may be of interest.

    The other documents are:

    A Primer on next-gen sequencing: A write-up on next-gen sequencing technologies to give an overview of what the state of play is.

    The Solexa Pipeline: This document describes how the Illumina/Solexa primary data analysis pipeline works at the algorithmic level (mostly image analysis).

    Information Content of ABI SOLiD 2 base encoded reads: Running the numbers on 2 base encoding.

    Early cycle errors in run 241: Looking at errors in an early GA1 run.

    Quality conversion sheet: Convert between Solexa and Phred Q values.

    All here: http://sgenomics.org/mediawiki/index.php/Genomics

    Comments, suggestions, requests for tex files etc. all welcome.

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