Hi,
My data is in a new format generated by the new illumina pipeline software v 1.8. So, the quality scores are in Sanger FASTQ format with ASCII offset 33.
I used following code of Fastx tooklit to find quality stats of my data.
root@SNP215-01:Cold_Shock_Filtered# fastx_quality_stats -Q33 -i Cold_Shock_negative_2.fq -o Cold_Shock_negative_2.fq.stats
I received the following ERROR message:
fastx_quality_stats: Invalid quality score value (char 'J' ord 74 quality value 41) on line 20
So, please help me to resolve this problem.
Thank you for your time !
Vedbar
My data is in a new format generated by the new illumina pipeline software v 1.8. So, the quality scores are in Sanger FASTQ format with ASCII offset 33.
I used following code of Fastx tooklit to find quality stats of my data.
root@SNP215-01:Cold_Shock_Filtered# fastx_quality_stats -Q33 -i Cold_Shock_negative_2.fq -o Cold_Shock_negative_2.fq.stats
I received the following ERROR message:
fastx_quality_stats: Invalid quality score value (char 'J' ord 74 quality value 41) on line 20
So, please help me to resolve this problem.
Thank you for your time !
Vedbar
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