Hey guys,
I'm using glimmerHMM to predict genes from a genome. I'm curious about the concept of the negative strand. Take the example file below
GlimmerHMM
Sequence name: ABCDE
Sequence length: 580 bp
Predicted genes/exons
Gene Exon Strand Exon Exon Range Exon
# # Type Length
1 1 - Terminal 139 144 6
Since it lies on the negative strand, does it mean that the region begins at position 139 (on a 1 to 580 scale) moving forward or from position 580-139+1 = 442 going backwards?
Sorry if this is something trivial i've overlooked,
Thanks in advance,
-A
I'm using glimmerHMM to predict genes from a genome. I'm curious about the concept of the negative strand. Take the example file below
GlimmerHMM
Sequence name: ABCDE
Sequence length: 580 bp
Predicted genes/exons
Gene Exon Strand Exon Exon Range Exon
# # Type Length
1 1 - Terminal 139 144 6
Since it lies on the negative strand, does it mean that the region begins at position 139 (on a 1 to 580 scale) moving forward or from position 580-139+1 = 442 going backwards?

Sorry if this is something trivial i've overlooked,
Thanks in advance,
-A