Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • Adam Witney
    Junior Member
    • Mar 2011
    • 7

    Newbler cannot use a "project lock file"?

    Hi,

    I have been using Newbler (runAssembly) with no problems on one server and all the files and the binary are in fact stored on an NFS.

    I am now trying to run it on a second server, also attached to the same NFS (so all the files/binary are the same), however I get this error:

    $ runAssembly -o Newbler data11.sff
    Error: Unable to create project directory: Newbler
    Could not use a project lock file.
    Error in runAssembly: Error while creating the assembly project.
    Usage: runAssembly [-o projdir] [-nrm] [-p (sfffile | [regionlist:]analysisDir)]... (sfffile | [regionlist:]analysisDir)...

    I presume it is having some permissions issue, but my username does have write permission, and before the error arrives it does in fact create the 'Newbler' directory. Does it try to write this "project lock file" somewhere else? Does anybody know where this lock file lives? or know what causes this error specifically?

    thanks

    adam
    Last edited by Adam Witney; 09-20-2011, 11:01 AM.
  • maubp
    Peter (Biopython etc)
    • Jul 2009
    • 1544

    #2
    Can you double check the NFS file system is mounted the same way on both machines?

    Comment

    • Adam Witney
      Junior Member
      • Mar 2011
      • 7

      #3
      Yes, they are both mounted:

      homes1:/homedirs8 on /homedirs8 type nfs (rw,soft,intr,bg,addr=IP)

      Comment

      • maubp
        Peter (Biopython etc)
        • Jul 2009
        • 1544

        #4
        The only time I've personally seen this was with a mounted drive, and I resorted to running it in a different directory.

        Perhaps this is down to the version of the library used to mount the drive... curious. Are the two servers running similar operating systems? e.g. Both the same Linux distribution?

        Comment

        • Adam Witney
          Junior Member
          • Mar 2011
          • 7

          #5
          one is CentOS 5.5 and the other CentOS 5.6, so pretty similar. I will talk to our sysadmin tomorrow to see if he might know of any differences between the mountings

          thanks

          adam

          Comment

          Latest Articles

          Collapse

          • GATTACAT
            Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
            by GATTACAT
            Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
            07-01-2026, 11:43 AM
          • SEQadmin2
            Nine Things a Sample Prep Scientist Thinks About Before Sequencing
            by SEQadmin2


            I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

            Here are nine questions we think about, in roughly the order they matter, before...
            06-18-2026, 07:11 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by SEQadmin2, 07-02-2026, 11:08 AM
          0 responses
          16 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 06-30-2026, 05:37 AM
          0 responses
          17 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 06-26-2026, 11:10 AM
          0 responses
          20 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 06-17-2026, 06:09 AM
          0 responses
          54 views
          0 reactions
          Last Post SEQadmin2  
          Working...