Hi all,
after comparing a newbler assembly with a reference genome I have identified two contigs which contain the beginning and end (respectively) of the same gene. A PCR was run to bridge the gap and the product was sequenced yielding a 790bp fragment which overlaps both contigs
I am curious to know which tool is best to close the gap between the contigs?
regards
Brian
after comparing a newbler assembly with a reference genome I have identified two contigs which contain the beginning and end (respectively) of the same gene. A PCR was run to bridge the gap and the product was sequenced yielding a 790bp fragment which overlaps both contigs
I am curious to know which tool is best to close the gap between the contigs?
regards
Brian
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