Importing Genome in IGB and Chip Seq data in IGV

[ayushraman]

Does any body knows how to import Genomes like T. brucei (which is not present in IGB browser) into IGB browser ?

Also, I tried using IGV (which has this genome) but when I loaded the ChIP-Seq Wig file (output from MACS) in the IGV, it doesn't show me any peaks. Any advice or hints will be highly appreciated.

[Jim Robinson]

Hi, does the wig file end with a ".wig" extension? Another problem might be a mismatch in chromosome / contig names. Finally, if the file is large you will be happier the wig to "TDF" format with igvtools, or bigwig.

If you want to post a few lines of the file I can advise further.

Jim

Does any body knows how to import Genomes like T. brucei (which is not present in IGB browser) into IGB browser ?

Also, I tried using IGV (which has this genome) but when I loaded the ChIP-Seq Wig file (output from MACS) in the IGV, it doesn't show me any peaks. Any advice or hints will be highly appreciated.