Combine multiple wigs into one bigwig

[biznatch]

I've got fixed step .wig files, 1 per chromosome, and I want to combine them into one bigwig file. I was going to concatenate them then use wigtobigwig. Step 2 of the instructions for creating a bigwig file says "Remove any existing 'track' or 'browser' lines from your wig file so that it contains only data." If I remove all the track lines then how will the wigtobigwig program know which data is on which chromosome? If I combine all my wig files then remove all the track lines it'll just be a file with a single column of numbers and no references to chromosomes. I think I'm missing something here...

Edit: I think I found the answer, someone else asking the same thing, I think the UCSC instructions need to be a bit clearer as even the UCSC "tech support" person was a little mixed up at first: https://lists.soe.ucsc.edu/pipermail...er/027040.html

[Orr Shomroni]

Personally, I'm using multiBamCov (a command in Bedtools) which converts multiple BAM files into BED, and piping it directly into wigToBigWig. This works since BED and WIG are of very similar format