I have the same question:which method should i choose?
Originally posted by umnklang
View Post
-
DEGseq calculation method
I have a question about using DEGseq. I am using it to calculation differentially expressed genes in bacteria. I have Illumina data that I have calculated RPKM from using perl scripts.
I feed that data into the DEGexp command and set rawCounts=False. I am wondering about the method = "?". Right now I use FET, but I'm not sure at all if this is correct. How does one go about choosing a method?
-K
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Leave a comment: