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  • umnklang
    Member
    • Oct 2011
    • 16

    #1

    DEGseq calculation method

    I have a question about using DEGseq. I am using it to calculation differentially expressed genes in bacteria. I have Illumina data that I have calculated RPKM from using perl scripts.

    I feed that data into the DEGexp command and set rawCounts=False. I am wondering about the method = "?". Right now I use FET, but I'm not sure at all if this is correct. How does one go about choosing a method?

    -K
  • wangxj
    Junior Member
    • Sep 2012
    • 7

    #2
    Hello,

    I have the same question:which method should i choose?

    Originally posted by umnklang View Post
    I have a question about using DEGseq. I am using it to calculation differentially expressed genes in bacteria. I have Illumina data that I have calculated RPKM from using perl scripts.

    I feed that data into the DEGexp command and set rawCounts=False. I am wondering about the method = "?". Right now I use FET, but I'm not sure at all if this is correct. How does one go about choosing a method?

    -K

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