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  • Is there any public raw intensity data from RNA-Seq

    Hi,

    I am wondering if there is any public available raw intensity data from RNA-seq before the base calling. For my previous RNA-seq data analysis, I usually starts with fasta/fastq fomat, where I have information for each base and its quality score. Recently, I am investigating some model which makes use of the raw intensity data. If I understand it correctly, before base calling and after obtaining raw image data, there should be some intermediate steps where we can have the intensity/prob. distribution on each possible nucleotide on each base. I have googled related topics and found that ".bcl" files might be what I am looking for (please correct me if I am wrong), and I am wondering if there is any public available data as what I described.

    Thanks,
    Zheng
    Last edited by endether; 10-10-2011, 08:15 AM.

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