Hi,
I am wondering if there is any public available raw intensity data from RNA-seq before the base calling. For my previous RNA-seq data analysis, I usually starts with fasta/fastq fomat, where I have information for each base and its quality score. Recently, I am investigating some model which makes use of the raw intensity data. If I understand it correctly, before base calling and after obtaining raw image data, there should be some intermediate steps where we can have the intensity/prob. distribution on each possible nucleotide on each base. I have googled related topics and found that ".bcl" files might be what I am looking for (please correct me if I am wrong), and I am wondering if there is any public available data as what I described.
Thanks,
Zheng
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
Latest Articles
Collapse
-
by seqadmin
The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
Channel: Articles
07-08-2024, 03:19 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 07-25-2024, 06:46 AM
|
0 responses
9 views
0 likes
|
Last Post
by seqadmin
07-25-2024, 06:46 AM
|
||
Started by seqadmin, 07-24-2024, 11:09 AM
|
0 responses
26 views
0 likes
|
Last Post
by seqadmin
07-24-2024, 11:09 AM
|
||
Started by seqadmin, 07-19-2024, 07:20 AM
|
0 responses
160 views
0 likes
|
Last Post
by seqadmin
07-19-2024, 07:20 AM
|
||
Started by seqadmin, 07-16-2024, 05:49 AM
|
0 responses
127 views
0 likes
|
Last Post
by seqadmin
07-16-2024, 05:49 AM
|