Hi,
Does anyone have any experience using Bowtie2 with Tophat?
If so could you send me your tophat script?
Does anyone have any experience using Bowtie2 with Tophat?
If so could you send me your tophat script?
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Never mind. I figured it out. -
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I posted that as i got it running.. It eventually core dumped and I spoke with the designers of tophat and they said it will take serious conversions, since bwt2 uses only sam output files instead of the bwtout as before and the -un command no longer exists and the unaligned reads are in the bam file. I decided I was better off waiting for them to release an update.Comment
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Did they say they will make an update of Tophat using Bowtie2?
It would be great, so long as the Tophat output can provide accurate Mapping Quality (MAPQ field in SAM format), which Bowtie2 does...Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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