There are so many software to mapping the solexa/SOLid reads to reference. Which is the best?
-
-
If you wont to align against a reference sequence more or less all the software are pretty good. They differ a bit from what concern speed and sensitivity but more or less are similar. In my lab we use our own tool that is more sensitive then ELAND and it has performances similar to BOWTIE an SOAP.Originally posted by anyone1985 View Post
For what concern assembly the best software is VELVET. I use also EULER-SR that is some cases perform better then VELVET.
Also EDANA is pretty good but VELVET and EULER are better... -
As a biased alignment tool author, I would suggest BFAST for indels and sensitivity.Originally posted by anyone1985 View Post
Comment
-
Thanks. When I assembled my data, I found the velvet was pretty good. And, I also tried to extend the sequences by different aligment. I would like to find more software with various ideas to help me finish my genome.
Originally posted by francesco.vezzi View PostComment
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment