a question about merge bam files

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camelbbs

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#1

a question about merge bam files

10-21-2011, 01:20 PM

I want to ask a question about bam files.

I have 2 sequencing library in a same sample, and get 2 fastq files, the length of reads are 50bp and 36bp separately.
When I do tophat, because I need to specify the -r, I cannot merge the two fastq files. But after I got the accepted.bam files, can I merge them (bam files) with the samtools merge?

I need to do cufflinks and cuffdiff using the merged bam files.

thanks everyone.
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kentnf

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#2

10-22-2011, 01:23 AM

I have the same question.
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ECO

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#3

10-24-2011, 09:00 AM

This question was crossposted here: http://seqanswers.com/forums/showthread.php?t=14980
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