Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • A Problem of BFAST with Open Tmp GZFile

    Hi, I have a weird problem with the bfast match step on CentOS 5.5, when I tried the following command:

    bfast mathch -f ucsc_hg19.fasta -r 17915_L1_1.fq -T /ifs2/user/bfast/tmp/

    and got this error:

    ************************************************************
    Reading 17915_L1_1.fq.gz into a temp file.
    ************************************************************
    In function "OpenTmpGZFile": Fatal Error[OpenFileError]. Variable/Value: /ifs2/user/bfast/tmp/.bfast.tmp.A2mYup.
    Message: Could not open temporary file.
    The file stream error was:: No such file or directory
    ***** Exiting due to errors *****
    ************************************************************

    We use the latest version of bfast(0.6.5a), and the commands to creat index are as follow:

    bfast fasta2brg -f ucsc_hg19.fasta -A 0
    bfast index -f ucsc_hg19.fasta -m 1111111111111111111111 -w 14 -i 1 -A 0 -t

    It is not about the writing rights since I changed the direction to full wrinting permissions(777), nor about the space of the filesystem. I entered the temporary direction, no file has been created.

    Is there anyone faced the same problem and found a solution?
    Thanks in advance!

  • #2
    Have you tried checking the BFAST mailing list ([email protected])?

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-25-2024, 11:49 AM
    0 responses
    19 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-24-2024, 08:47 AM
    0 responses
    20 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    62 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    61 views
    0 likes
    Last Post seqadmin  
    Working...
    X