Hi all,
I am a new user of the next generation sequencing.
I am working with Illumina output sequence reads of a non-model organism so I have not a reference genome. I would like to quantify gene expression of diffrent group of individuals through the CLC Workbench (mRNA sequencing tool based on the RPKM concept). Do you think can I use the the assembled contigs as a reference genome and use this tool to compare gene expression in different individuals?
Thanks!!!
P
I am a new user of the next generation sequencing.
I am working with Illumina output sequence reads of a non-model organism so I have not a reference genome. I would like to quantify gene expression of diffrent group of individuals through the CLC Workbench (mRNA sequencing tool based on the RPKM concept). Do you think can I use the the assembled contigs as a reference genome and use this tool to compare gene expression in different individuals?
Thanks!!!
P
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